gs 700 imaging densitometer Search Results


96
LI-COR revert total protein stain
Autophagy is upregulated in caf mutants but 3-MA treatment does not improve the swimming behavior or dystrophic phenotype of caf mutants. (A) Confocal micrographs of whole-mount embryos <t>stained</t> with anti-LC3 (green), anti-Dystrophin (orange), Phalloidin (magenta) and DAPI (blue). LC3 localization and abundance are affected in caf mutants at 5 dpf; LC3 accumulates at sites of fiber detachment (white arrowheads). (B) Western blot analysis showing increased levels of LC3 II in caf mutants. LC3 II levels were normalized against <t>total</t> <t>protein.</t> (C) Western blot analysis showing decreased levels of LC3 II in WT zebrafish after 3-MA treatment. LC3 II levels were normalized against β-actin. (D–F) Swimming behavior analysis. There was no significant change at 3 dpf in time spent moving (D), total distance traveled (E) or average velocity (F) in caf mutants after treatment with 10 mM 3-MA (n = 165). Bars represent mean ± SEM.
Revert Total Protein Stain, supplied by LI-COR, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Gatan Inc digital micrograph
Autophagy is upregulated in caf mutants but 3-MA treatment does not improve the swimming behavior or dystrophic phenotype of caf mutants. (A) Confocal micrographs of whole-mount embryos <t>stained</t> with anti-LC3 (green), anti-Dystrophin (orange), Phalloidin (magenta) and DAPI (blue). LC3 localization and abundance are affected in caf mutants at 5 dpf; LC3 accumulates at sites of fiber detachment (white arrowheads). (B) Western blot analysis showing increased levels of LC3 II in caf mutants. LC3 II levels were normalized against <t>total</t> <t>protein.</t> (C) Western blot analysis showing decreased levels of LC3 II in WT zebrafish after 3-MA treatment. LC3 II levels were normalized against β-actin. (D–F) Swimming behavior analysis. There was no significant change at 3 dpf in time spent moving (D), total distance traveled (E) or average velocity (F) in caf mutants after treatment with 10 mM 3-MA (n = 165). Bars represent mean ± SEM.
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90
Panasonic Healthcare ccd camera with intensifier panasonic wv-cl 700/a
Autophagy is upregulated in caf mutants but 3-MA treatment does not improve the swimming behavior or dystrophic phenotype of caf mutants. (A) Confocal micrographs of whole-mount embryos <t>stained</t> with anti-LC3 (green), anti-Dystrophin (orange), Phalloidin (magenta) and DAPI (blue). LC3 localization and abundance are affected in caf mutants at 5 dpf; LC3 accumulates at sites of fiber detachment (white arrowheads). (B) Western blot analysis showing increased levels of LC3 II in caf mutants. LC3 II levels were normalized against <t>total</t> <t>protein.</t> (C) Western blot analysis showing decreased levels of LC3 II in WT zebrafish after 3-MA treatment. LC3 II levels were normalized against β-actin. (D–F) Swimming behavior analysis. There was no significant change at 3 dpf in time spent moving (D), total distance traveled (E) or average velocity (F) in caf mutants after treatment with 10 mM 3-MA (n = 165). Bars represent mean ± SEM.
Ccd Camera With Intensifier Panasonic Wv Cl 700/A, supplied by Panasonic Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LI-COR odyssey clx fluorescence imaging system
Autophagy is upregulated in caf mutants but 3-MA treatment does not improve the swimming behavior or dystrophic phenotype of caf mutants. (A) Confocal micrographs of whole-mount embryos <t>stained</t> with anti-LC3 (green), anti-Dystrophin (orange), Phalloidin (magenta) and DAPI (blue). LC3 localization and abundance are affected in caf mutants at 5 dpf; LC3 accumulates at sites of fiber detachment (white arrowheads). (B) Western blot analysis showing increased levels of LC3 II in caf mutants. LC3 II levels were normalized against <t>total</t> <t>protein.</t> (C) Western blot analysis showing decreased levels of LC3 II in WT zebrafish after 3-MA treatment. LC3 II levels were normalized against β-actin. (D–F) Swimming behavior analysis. There was no significant change at 3 dpf in time spent moving (D), total distance traveled (E) or average velocity (F) in caf mutants after treatment with 10 mM 3-MA (n = 165). Bars represent mean ± SEM.
Odyssey Clx Fluorescence Imaging System, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Bio-Rad gs 700 imaging densitometer
Autophagy is upregulated in caf mutants but 3-MA treatment does not improve the swimming behavior or dystrophic phenotype of caf mutants. (A) Confocal micrographs of whole-mount embryos <t>stained</t> with anti-LC3 (green), anti-Dystrophin (orange), Phalloidin (magenta) and DAPI (blue). LC3 localization and abundance are affected in caf mutants at 5 dpf; LC3 accumulates at sites of fiber detachment (white arrowheads). (B) Western blot analysis showing increased levels of LC3 II in caf mutants. LC3 II levels were normalized against <t>total</t> <t>protein.</t> (C) Western blot analysis showing decreased levels of LC3 II in WT zebrafish after 3-MA treatment. LC3 II levels were normalized against β-actin. (D–F) Swimming behavior analysis. There was no significant change at 3 dpf in time spent moving (D), total distance traveled (E) or average velocity (F) in caf mutants after treatment with 10 mM 3-MA (n = 165). Bars represent mean ± SEM.
Gs 700 Imaging Densitometer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Carl Zeiss lsm 710 spectral confocal laser scanning microscope
Autophagy is upregulated in caf mutants but 3-MA treatment does not improve the swimming behavior or dystrophic phenotype of caf mutants. (A) Confocal micrographs of whole-mount embryos <t>stained</t> with anti-LC3 (green), anti-Dystrophin (orange), Phalloidin (magenta) and DAPI (blue). LC3 localization and abundance are affected in caf mutants at 5 dpf; LC3 accumulates at sites of fiber detachment (white arrowheads). (B) Western blot analysis showing increased levels of LC3 II in caf mutants. LC3 II levels were normalized against <t>total</t> <t>protein.</t> (C) Western blot analysis showing decreased levels of LC3 II in WT zebrafish after 3-MA treatment. LC3 II levels were normalized against β-actin. (D–F) Swimming behavior analysis. There was no significant change at 3 dpf in time spent moving (D), total distance traveled (E) or average velocity (F) in caf mutants after treatment with 10 mM 3-MA (n = 165). Bars represent mean ± SEM.
Lsm 710 Spectral Confocal Laser Scanning Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss confocal microscopy zeiss lsm510
Autophagy is upregulated in caf mutants but 3-MA treatment does not improve the swimming behavior or dystrophic phenotype of caf mutants. (A) Confocal micrographs of whole-mount embryos <t>stained</t> with anti-LC3 (green), anti-Dystrophin (orange), Phalloidin (magenta) and DAPI (blue). LC3 localization and abundance are affected in caf mutants at 5 dpf; LC3 accumulates at sites of fiber detachment (white arrowheads). (B) Western blot analysis showing increased levels of LC3 II in caf mutants. LC3 II levels were normalized against <t>total</t> <t>protein.</t> (C) Western blot analysis showing decreased levels of LC3 II in WT zebrafish after 3-MA treatment. LC3 II levels were normalized against β-actin. (D–F) Swimming behavior analysis. There was no significant change at 3 dpf in time spent moving (D), total distance traveled (E) or average velocity (F) in caf mutants after treatment with 10 mM 3-MA (n = 165). Bars represent mean ± SEM.
Confocal Microscopy Zeiss Lsm510, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss lsm 700/880
Autophagy is upregulated in caf mutants but 3-MA treatment does not improve the swimming behavior or dystrophic phenotype of caf mutants. (A) Confocal micrographs of whole-mount embryos <t>stained</t> with anti-LC3 (green), anti-Dystrophin (orange), Phalloidin (magenta) and DAPI (blue). LC3 localization and abundance are affected in caf mutants at 5 dpf; LC3 accumulates at sites of fiber detachment (white arrowheads). (B) Western blot analysis showing increased levels of LC3 II in caf mutants. LC3 II levels were normalized against <t>total</t> <t>protein.</t> (C) Western blot analysis showing decreased levels of LC3 II in WT zebrafish after 3-MA treatment. LC3 II levels were normalized against β-actin. (D–F) Swimming behavior analysis. There was no significant change at 3 dpf in time spent moving (D), total distance traveled (E) or average velocity (F) in caf mutants after treatment with 10 mM 3-MA (n = 165). Bars represent mean ± SEM.
Lsm 700/880, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Carl Zeiss lsm 700 confocal laser scanning microscope
Autophagy is upregulated in caf mutants but 3-MA treatment does not improve the swimming behavior or dystrophic phenotype of caf mutants. (A) Confocal micrographs of whole-mount embryos <t>stained</t> with anti-LC3 (green), anti-Dystrophin (orange), Phalloidin (magenta) and DAPI (blue). LC3 localization and abundance are affected in caf mutants at 5 dpf; LC3 accumulates at sites of fiber detachment (white arrowheads). (B) Western blot analysis showing increased levels of LC3 II in caf mutants. LC3 II levels were normalized against <t>total</t> <t>protein.</t> (C) Western blot analysis showing decreased levels of LC3 II in WT zebrafish after 3-MA treatment. LC3 II levels were normalized against β-actin. (D–F) Swimming behavior analysis. There was no significant change at 3 dpf in time spent moving (D), total distance traveled (E) or average velocity (F) in caf mutants after treatment with 10 mM 3-MA (n = 165). Bars represent mean ± SEM.
Lsm 700 Confocal Laser Scanning Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss lsm 700 laser scanning microscope
Autophagy is upregulated in caf mutants but 3-MA treatment does not improve the swimming behavior or dystrophic phenotype of caf mutants. (A) Confocal micrographs of whole-mount embryos <t>stained</t> with anti-LC3 (green), anti-Dystrophin (orange), Phalloidin (magenta) and DAPI (blue). LC3 localization and abundance are affected in caf mutants at 5 dpf; LC3 accumulates at sites of fiber detachment (white arrowheads). (B) Western blot analysis showing increased levels of LC3 II in caf mutants. LC3 II levels were normalized against <t>total</t> <t>protein.</t> (C) Western blot analysis showing decreased levels of LC3 II in WT zebrafish after 3-MA treatment. LC3 II levels were normalized against β-actin. (D–F) Swimming behavior analysis. There was no significant change at 3 dpf in time spent moving (D), total distance traveled (E) or average velocity (F) in caf mutants after treatment with 10 mM 3-MA (n = 165). Bars represent mean ± SEM.
Lsm 700 Laser Scanning Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss clsm lsm 700
Autophagy is upregulated in caf mutants but 3-MA treatment does not improve the swimming behavior or dystrophic phenotype of caf mutants. (A) Confocal micrographs of whole-mount embryos <t>stained</t> with anti-LC3 (green), anti-Dystrophin (orange), Phalloidin (magenta) and DAPI (blue). LC3 localization and abundance are affected in caf mutants at 5 dpf; LC3 accumulates at sites of fiber detachment (white arrowheads). (B) Western blot analysis showing increased levels of LC3 II in caf mutants. LC3 II levels were normalized against <t>total</t> <t>protein.</t> (C) Western blot analysis showing decreased levels of LC3 II in WT zebrafish after 3-MA treatment. LC3 II levels were normalized against β-actin. (D–F) Swimming behavior analysis. There was no significant change at 3 dpf in time spent moving (D), total distance traveled (E) or average velocity (F) in caf mutants after treatment with 10 mM 3-MA (n = 165). Bars represent mean ± SEM.
Clsm Lsm 700, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Autophagy is upregulated in caf mutants but 3-MA treatment does not improve the swimming behavior or dystrophic phenotype of caf mutants. (A) Confocal micrographs of whole-mount embryos stained with anti-LC3 (green), anti-Dystrophin (orange), Phalloidin (magenta) and DAPI (blue). LC3 localization and abundance are affected in caf mutants at 5 dpf; LC3 accumulates at sites of fiber detachment (white arrowheads). (B) Western blot analysis showing increased levels of LC3 II in caf mutants. LC3 II levels were normalized against total protein. (C) Western blot analysis showing decreased levels of LC3 II in WT zebrafish after 3-MA treatment. LC3 II levels were normalized against β-actin. (D–F) Swimming behavior analysis. There was no significant change at 3 dpf in time spent moving (D), total distance traveled (E) or average velocity (F) in caf mutants after treatment with 10 mM 3-MA (n = 165). Bars represent mean ± SEM.

Journal: Human Molecular Genetics

Article Title: Lysosomes and the pathogenesis of merosin-deficient congenital muscular dystrophy

doi: 10.1093/hmg/ddab278

Figure Lengend Snippet: Autophagy is upregulated in caf mutants but 3-MA treatment does not improve the swimming behavior or dystrophic phenotype of caf mutants. (A) Confocal micrographs of whole-mount embryos stained with anti-LC3 (green), anti-Dystrophin (orange), Phalloidin (magenta) and DAPI (blue). LC3 localization and abundance are affected in caf mutants at 5 dpf; LC3 accumulates at sites of fiber detachment (white arrowheads). (B) Western blot analysis showing increased levels of LC3 II in caf mutants. LC3 II levels were normalized against total protein. (C) Western blot analysis showing decreased levels of LC3 II in WT zebrafish after 3-MA treatment. LC3 II levels were normalized against β-actin. (D–F) Swimming behavior analysis. There was no significant change at 3 dpf in time spent moving (D), total distance traveled (E) or average velocity (F) in caf mutants after treatment with 10 mM 3-MA (n = 165). Bars represent mean ± SEM.

Article Snippet: Total protein was stained with Revert Total Protein Stain (LI-COR Biotechnology; Lincoln, NE, USA) and visualized with Odyssey FX Imaging system using Image Studio software (LI-COR Biotechnology).

Techniques: Staining, Western Blot